• Product nameAnti-HLA DP antibody
    See all HLA DP primary antibodies
  • Description
    Mouse polyclonal to HLA DP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-260 of human HLA DP (AAH09956.1).

  • Positive control
    • Human spleen, transfected 293T cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab88089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.


  • RelevanceHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
  • Cellular localizationType I membrane protein; Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • Database links
  • Alternative names
    • DP(W3) antibody
    • DP(W4) antibody
    • HLA class II histocompatibility antigen DP alpha 1 chain antibody
    • HLA class II histocompatibility antigen DP alpha chain antibody
    • HLA DP1A antibody
    • HLA DPA 1 antibody
    • HLA SB alpha chain antibody
    • HLADP antibody
    • HLADPA1 antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha gene antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • MHC class II antigen antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA DPA1 antigen antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    see all

Anti-HLA DP antibody images

  • Anti-HLA DP antibody (ab88089) at 1 µg/ml + human spleen lysate at 50 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HLA DP antibody (ab88089) at 1 µg/ml

    Lane 1 : transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa

References for Anti-HLA DP antibody (ab88089)

ab88089 has not yet been referenced specifically in any publications.

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Here is a link to the fractionation protocol as discussed: http://www.abcam.com/index.html?pageconfig=resource&rid=11473 I really think that enriching for the membrane fraction may help. I will also discuss with one of my colleagues.

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