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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab20897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|Inhibition Assay||Use at an assay dependent concentration. PubMed: 19531622|
|ICC/IF||Use a concentration of 5 µg/ml.|
Raw 246.7 cells stained for HLA-DP (colored green) using ab20897 in ICC/IF. The cells were fixed with 4% formaldehyde (10 min) then permeabilized using 0.1% Triton X-100 in PBS buffer and then incubated in 1% bovine serum albumin (BSA) + 10% normal goat serum + 0.3 M glycine + 0.1% Tween-20 in PBS buffer for 1 hour to further permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the HLA-DP antibody ab20897 at 5 µg/ml overnight at +4°C. The secondary antibody (colored green) was Alexa Fluor® 488 goat anti-mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.
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