HLA DP overexpression 293T lysate (whole cell) (ab94190)

Overview

  • Product nameHLA DP overexpression 293T lysate (whole cell)
  • Description
    HLA DP overexpression lysate
  • General notesab94190 is a 293T cell transfected lysate in which Human HLA DP has been transiently over-expressed using a pCMV-HLS DP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

HLA DP overexpression 293T lysate (whole cell) images

  • ab94190 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-HLA DP antibody (ab88089) at 1/500 dilution

    Lane 1 : HLA DP overexpression 293T lysate (whole cell) (ab94190)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for HLA DP overexpression 293T lysate (whole cell) (ab94190)

ab94190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"