Overview

  • Product nameAnti-HLA DQ antibody [SPV-L3]
    See all HLA DQ primary antibodies
  • Description
    Mouse monoclonal [SPV-L3] to HLA DQ
  • Specificityab23632 reacts with a monomorphic determinant of human class II HLA DQ molecules and strongly blocks cytotoxicity. Distribution studies suggest that it is directed against DQ rather than DR antigens. Clone SPV-L3 has been reported to inhibit the activity of cytotoxic T cell clones.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Inhibition Assay, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Cells of T4+ CTL clone HG-38.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 10mM Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberSPV-L3
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. PubMed: 15795275

Removal of sodium azide is recommended prior to use in functional assays.

Target

  • RelevanceHuman Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens: class I (HLA A, HLA B and HLA C) and class II (HLA D).
  • Cellular localizationType I membrane protein
  • Database links
  • Alternative names
    • CD antibody
    • CELIAC1 antibody
    • DC 4 alpha chain antibody
    • DC alpha antibody
    • DQ A1 antibody
    • Gluten sensitive enteropathy (celiac disease) antibody
    • GSE antibody
    • HLA Class II DQ antibody
    • HLA class II histocompatibility antigen DQ antibody
    • HLA class II histocompatibility antigen DQ(1) alpha chain antibody
    • HLA class II histocompatibility antigen DQ(3) alpha chain antibody
    • HLA DCA antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Leucocyte antigen DQA1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • MHC class II antigen HLA DQ beta 1 antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DQ beta glycoprotein antibody
    • MHC DQ beta antibody
    • MHC HLA DQ alpha antibody
    see all

References for Anti-HLA DQ antibody [SPV-L3] (ab23632)

This product has been referenced in:
  • Fairfax BP  et al. Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression. Science 343:1246949 (2014). Flow Cyt ; Human . Read more (PubMed: 24604202) »
  • Lapaque N  et al. Salmonella regulates polyubiquitination and surface expression of MHC class II antigens. Proc Natl Acad Sci U S A 106:14052-7 (2009). Flow Cyt ; Human . Read more (PubMed: 19666567) »

See all 2 Publications for this product

Product Wall

Thank you very much for your reply.

The binding affinity and mechanism varies between blocking antibodies, butthey do seem to generally act as antagonists. These kinds of characteristics need to be determined for each individual antibody and ...

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Thank you again for contacting us and for your patience while I've been in touch with the lab about your enquiry.

Ab20897 against HLA-DP has not been tested in a blocking experiment at this time, though it is possible that it will work in suc...

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Thank you very much for contacting us with your enquiry.

Theterm "functional studies" can be used to describe several different kinds of assays, and sometimes blocking assays are included in this categorization so there isn't a spec...

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Thank you for your enquiry. This product was produced by immunising mice with the HG-38 Cell line. Our QC lab uses either western blot or flowcytometry to determine specificity. Finally, this clone SPV-L3 has been reported to inhibit the activity ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"