Anti-HLA DR + DP + DQ antibody [CR3/43] (ab17101)


  • Product nameAnti-HLA DR + DP + DQ antibody [CR3/43]
    See all HLA DR + DP + DQ primary antibodies
  • Description
    Mouse monoclonal [CR3/43] to HLA DR + DP + DQ
  • SpecificityThis antibody reacts with the beta chain of all products of the gene sub regions DP, DQ, and DR. It stains B cells, interdigitating reticulum cells, Langerhans cells and many macrophages. This antibody does not react with normal T cell and polymorphs but stains activated T cells.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • Positive control
    • Tonsil


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Tissue culture supernatant
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberCR3/43
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab17101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: 1/25 - 1/50, in an ABC method.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • Sequence similaritiesBelongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD antibody
      • CELIAC 1 antibody
      • CELIAC1 antibody
      • DP beta 1 chain antibody
      • DP(W4) beta chain antibody
      • DPB1 antibody
      • DPB1_HUMAN antibody
      • DQ A1 antibody
      • DRB1 antibody
      • DRB4 antibody
      • FLJ27088 antibody
      • FLJ27328 antibody
      • GSE antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen DR 1 beta antibody
      • HLA class II histocompatibility antigen DR alpha antibody
      • HLA class II histocompatibility antigen DR alpha chain antibody
      • HLA DP histocompatibility type beta 1 subunit antibody
      • HLA DP1B antibody
      • HLA DPB1 antibody
      • HLA DQA antibody
      • HLA DQA1 antibody
      • HLA DQB antibody
      • HLA DQB1 antibody
      • HLA DR1B antibody
      • HLA DR3B antibody
      • HLA DR4B antibody
      • HLA DRA antibody
      • HLA DRA1 antibody
      • HLA DRB1 antibody
      • HLA DRB3 antibody
      • HLA DRB4 antibody
      • HLA DRB5 antibody
      • HLA-DPB1 antibody
      • IDDM 1 antibody
      • IDDM1 antibody
      • Major histocompatibility complex class II DP beta 1 antibody
      • Major histocompatibility complex class II DQ alpha 1 antibody
      • Major histocompatibility complex class II DQ beta 1 antibody
      • Major histocompatibility complex class II DR alpha antibody
      • Major histocompatibility complex class II DR beta 1 antibody
      • Major histocompatibility complex class II DR beta 3 antibody
      • Major histocompatibility complex class II DR beta 4 antibody
      • Major histocompatibility complex class II DR beta 5 antibody
      • MGC117330 antibody
      • MHC class II antigen DPB1 antibody
      • MHC class II antigen DRA antibody
      • MHC class II HLA DQ alpha 1 antibody
      • MHC class II HLA DR beta 1 antibody
      • MHC class II HLA DR beta 3 antibody
      • MHC DPB1 antibody
      • MHC DQ beta antibody
      • MHC HLA DPB1 antibody
      • MHC HLA DQ alpha antibody
      see all

    Anti-HLA DR + DP + DQ antibody [CR3/43] images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining HLA DR + DP + DQ with ab17101.

    References for Anti-HLA DR + DP + DQ antibody [CR3/43] (ab17101)

    ab17101 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil/Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6 20 mins 100 oC
    Permeabilization No
    Specification Tonsil/Colon
    Blocking step No blocking step used for 10 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted May 30 2016


    Expiration date: 01/09/2012

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