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Burkitt lymphoma cell line Raji.
The antibody is useful for detection of HLA-DR and HLA-DP positive B-cells, T-cells and monocytes in cell suspensions, in frozen sections as well as in formalin fixed paraffin embedded tissue sections. The antibody is also useful for characterization of leukemias and lymphomas. In experimental settings it can be used for selective removal of antigen carrying cells by complement mediated cytotoxicity. The antibody has excellent staining properties in histology.
Our Abpromise guarantee covers the use of ab8085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. PubMed: 19862823|
|Flow Cyt||Use 0.01µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20959457|
|IP||Use at an assay dependent concentration.|
Human peripheral blood lymphocytes stained with ab8085 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8085, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.