Overview

  • Product nameAnti-HLA DR + HLA DP antibody [HL-40]
    See all HLA DR + HLA DP primary antibodies
  • Description
    Mouse monoclonal [HL-40] to HLA DR + HLA DP
  • SpecificityCommon epitope on b-chain of human HLA-DR and HLA-DP. Also reacts with porcine MHC class II antigens (SLA-D gene products).
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Burkitt lymphoma cell line Raji.

  • General notes


    The antibody is useful for detection of HLA-DR and HLA-DP positive B-cells, T-cells and monocytes in cell suspensions, in frozen sections as well as in formalin fixed paraffin embedded tissue sections. The antibody is also useful for characterization of leukemias and lymphomas. In experimental settings it can be used for selective removal of antigen carrying cells by complement mediated cytotoxicity. The antibody has excellent staining properties in histology.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein-A affinity chromatography.
  • Primary antibody notesThe antibody is useful for detection of HLA-DR and HLA-DP positive B-cells, T-cells and monocytes in cell suspensions, in frozen sections as well as in formalin fixed paraffin embedded tissue sections. The antibody is also useful for characterization of leukemias and lymphomas. In experimental settings it can be used for selective removal of antigen carrying cells by complement mediated cytotoxicity. The antibody has excellent staining properties in histology.
  • ClonalityMonoclonal
  • Clone numberHL-40
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 19862823
Flow Cyt Use 0.01µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20959457
IP Use at an assay dependent concentration.

Target

  • RelevanceHuman MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
  • Cellular localizationCell Membrane; Single pass type I membrane protein
  • Database links
  • Alternative names
    • HLA DP antibody
    • HLA DR antibody
    • MHC Class II antibody

Anti-HLA DR + HLA DP antibody [HL-40] images

  • Human peripheral blood lymphocytes stained with ab8085 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8085, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • HeLa cells were transfected with DR. The cells were fixed in paraformaldehyde and either left unpermeabilized (-Tx) or were permeabilized with 0.2% Triton X-100 (+Tx). DR- and DP-transfected cells were immunostained with ab8085. Nuclei were stained with DAPI.
  • HeLa cells were transfected with different combinations of a, ß, and Ii constructs as indicated. Lysates were first subjected to immunoprecipitation (IP) with ab8085 before Western blotting (WB) with anti-DP and anti-Ii. H and L indicate the heavy and light chains of the antibodies used for the immunoprecipitation.

References for Anti-HLA DR + HLA DP antibody [HL-40] (ab8085)

This product has been referenced in:
  • van Lith M  et al. HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM. J Biol Chem 285:40800-8 (2010). ICC/IF, IP ; Human . Read more (PubMed: 20959457) »
  • Degenhardt Y  et al. Distinct MHC gene expression patterns during progression of melanoma. Genes Chromosomes Cancer 49:144-54 (2010). WB ; Human . Read more (PubMed: 19862823) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. This antibody has not been tested for cross-reaction with sheep(ovine) antigens.

Yes WB was performed under reducing conditions. For most details on Western Blotting, please refer to Horejsi et al. Tissue Antigens 28, 288-291 (1986)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"