FITC Anti-HLA DR + HLA DP antibody [MEM-136] (ab77237)
Key features and details
- FITC Mouse monoclonal [MEM-136] to HLA DR + HLA DP
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
-
Product name
FITC Anti-HLA DR + HLA DP antibody [MEM-136]
See all HLA DR + HLA DP primary antibodies -
Description
FITC Mouse monoclonal [MEM-136] to HLA DR + HLA DP -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab77237 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human HLA DR + HLA DP. PHA-activated peripheral blood lymphocytes
-
Positive control
- Human blood cells.
-
General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% (w/v) high-grade protease free BSA -
Concentration information loading...
-
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-136 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 20µl for 106 cells or 100µl whole blood. |
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 20µl for 106 cells or 100µl whole blood. |
Target
-
Relevance
Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles. -
Cellular localization
Cell Membrane; Single pass type I membrane protein -
Database links
- Entrez Gene: 3115 Human
- Entrez Gene: 3122 Human
- Entrez Gene: 3123 Human
- Entrez Gene: 3125 Human
- Entrez Gene: 3126 Human
- Entrez Gene: 3127 Human
- Entrez Gene: 972 Human
- Omim: 142858 Human
see all -
Alternative names
- HLA DP antibody
- HLA DR antibody
- MHC Class II antibody
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab77237 has been referenced in 1 publication.
- Koch C et al. T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol 11:777-86 (1999). PubMed: 10330283