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Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Our Abpromise guarantee covers the use of ab11820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. PubMed: 20713594|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
ab11820 staining HLA E in Human endothelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 5% BSA in PBS with 0.1% Triton X-100) for 1 hour at 4°C. An undiluted FITC-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.
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