HLA E overexpression 293T lysate (whole cell) (ab94202)

Overview

  • Product nameHLA E overexpression 293T lysate (whole cell)
  • Description
    HLA E overexpression lysate
  • General notesab94202 is a 293T cell transfected lysate in which Human HLA E has been transiently over-expressed using a pCMV-HLA E plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

HLA E overexpression 293T lysate (whole cell) images

  • ab94202 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HLA E antibody (ab88090) at 1/500 dilution

    Lane 1 : HLA E overexpression 293T lysate (whole cell) (ab94202)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for HLA E overexpression 293T lysate (whole cell) (ab94202)

ab94202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"