Overview

  • Product nameAnti-HLA F antibody
    See all HLA F primary antibodies
  • Description
    Rabbit polyclonal to HLA F
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Chimpanzee, African Green Monkey
  • Immunogen

    A synthetic peptide corresponding to a region of human HLA F

  • Positive control
    • 721_B cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in the presentation of foreign antigens to the immune system.
  • Sequence similaritiesBelongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDA12 antibody
    • HLA 5.4 antibody
    • HLA class I histocompatibility antigen, alpha chain F antibody
    • HLA class I molecule antibody
    • HLA F antigen antibody
    • HLA-CDA12 antibody
    • HLA-F antibody
    • HLAF antibody
    • HLAF_HUMAN antibody
    • Leukocyte antigen F antibody
    • MHC class I antigen F antibody
    see all

Anti-HLA F antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HLA F with ab81070 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane and cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HLA F. Right - Merge.
  • Anti-HLA F antibody (ab81070) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-HLA F antibody (ab81070)

ab81070 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab81070.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"