Recombinant
RabMAb

Anti-HLA F antibody [EPR6803] (ab126624)

Overview

  • Product name
    Anti-HLA F antibody [EPR6803]
    See all HLA F primary antibodies
  • Description
    Rabbit monoclonal [EPR6803] to HLA F
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human HLA F (P30511).

  • Positive control
    • Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.52 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6803
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Involved in the presentation of foreign antigens to the immune system.
    • Sequence similarities
      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDA12 antibody
      • HLA 5.4 antibody
      • HLA class I histocompatibility antigen, alpha chain F antibody
      • HLA class I molecule antibody
      • HLA F antigen antibody
      • HLA-CDA12 antibody
      • HLA-F antibody
      • HLAF antibody
      • HLAF_HUMAN antibody
      • Leukocyte antigen F antibody
      • MHC class I antigen F antibody
      see all

    Images

    • ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.
    • All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution

      Lane 1 : HT1376 cell lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 39 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126624 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    Placenta
    Fixative
    Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Feb 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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