Biotin Anti-HLA G antibody [4H84] (ab52456)
Key features and details
- Biotin Mouse monoclonal [4H84] to HLA G
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-HLA G antibody [4H84]
See all HLA G primary antibodies -
Description
Biotin Mouse monoclonal [4H84] to HLA G -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HLA G aa 61-83.
Sequence:DSDSACPRMEPRAPWVEQEGPEY
Database link: P17693 -
Positive control
- WB: HLA-G1 transfectants (LCL-HLA-G1).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Prior to conjugation, ab52456 was purified by Protein A affinity chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4H84 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
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All lanes : Biotin Anti-HLA G antibody [4H84] (ab52456) at 2 µg/ml
Lane 1 : JEG-3 red
Lane 2 : LNCaP red
Lane 3 : JEG-3 non-red
Lane 4 : LNCap non-red
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Following biotinylated mouse anti-HLA-G monoclonal antibody (ab52456) incubation, IRDye800-conjugated streptavidin was added to the nitrocellulose membrane.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab52456 has been referenced in 1 publication.
- Moreau P et al. HLA-G gene repression is reversed by demethylation. Proc Natl Acad Sci U S A 100:1191-6 (2003). WB, ICC ; Human . PubMed: 12552087