Overview

  • Product nameAnti-HLA G antibody [MEM-G/1]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/1] to HLA G
  • SpecificityThe antibody reacts with denaturated HLA-G heavy chain.
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human, Macaque Monkey
  • Immunogen

    Recombinant full length denatured heavy chain (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites using protein A affinity chromatography. Purity: >95% (by PAGE).
  • ClonalityMonoclonal
  • Clone numberMEM-G/1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 38.2 kDa. Does not react with native HLA-G on cell surface.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionInvolved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificityExpressed in trophoblasts.
  • Sequence similaritiesBelongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [MEM-G/1] images

  • The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
  • All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 38.2 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HLA G antibody [MEM-G/1] (ab7759)

This product has been referenced in:
  • Le Page ME  et al. Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-? responses to soluble HLA-G preparations. J Immunol 192:732-40 (2014). Read more (PubMed: 24337374) »
  • Vasireddi M & Hilliard J Herpes B virus, macacine herpesvirus 1, breaks simplex virus tradition via major histocompatibility complex class I expression in cells from human and macaque hosts. J Virol 86:12503-11 (2012). WB ; Human, Macaque Monkey . Read more (PubMed: 22973043) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Specification Placenta
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Feb 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (JEG-3)
Loading amount 30 µg
Specification JEG-3
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Jun 01 2010

Thank you for your enquiry. I can confirm that the recombinant protein was produced in bacteria. If there is anything else that I can help you with, please do not hesitate to contact me.

Thank you for your enquiry. I would recommend a starting dilution of 1/100, a concentration of 10 ug/mL, for this antibody in IHC-P. It is quite common to use a concentration between 1-20 ug/mL for IHC experiments. For this particular antibody we ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Human Tissue sections (Placenta, extravillous cytotrophoblasts)
Specification Placenta, extravillous cytotrophoblasts
Username

Mr. Richard Bruggeman

Verified customer

Submitted Jun 30 2005

Thank you for your enquiry regarding ab7759. The product datasheet for this antibody can be viewed by logging onto our website at www.Abcam.com and entering ab7759 in the search field. All relevant information such as price, technical data and availabi...

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Thank you for your enquiry and your interest in our products. We would like to confirm that ab7759 is a monoclonal antibody which recognizes human HLA-G1 and MEM-G/1 is the clone number. Please take a look at the on-line datasheet of this produc...

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HLA-G1 usually moves as 39 kDa band. Other isoforms (G2, G3, G4, G5,) are also detected if present in your sample.

Try this antibody at 1:100 and adjust accordingly from the results obtained. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6.0, 15 min.

This antibody reacts with denaturated heavy chain of HLA-G. Routine immunohistochemical protocol for staining of formalin-fixed, paraffin embedded sections is applicable. Recommended epitope retrieval is performed by microwave heating in citrate buffer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"