Anti-HLA G antibody [MEM-G/1] (ab7759)
Key features and details
- Mouse monoclonal [MEM-G/1] to HLA G
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-HLA G antibody [MEM-G/1]
See all HLA G primary antibodies -
Description
Mouse monoclonal [MEM-G/1] to HLA G -
Host species
Mouse -
Specificity
The antibody reacts with denaturated HLA-G heavy chain. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length denatured heavy chain (Human).
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Positive control
- IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-G/1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.
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Notes |
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IHC-P
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa. |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
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Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).
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All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 38.2 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands. -
The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
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All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCap cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCap cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody
Predicted band size: 38.2 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Western blotting analysis of human HLA-G using ab7759 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab7759 followed by IRDye800-conjugated anti-mouse secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab7759 has been referenced in 25 publications.
- Wang LJ et al. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation. Nat Commun 13:1626 (2022). PubMed: 35338152
- Bi Y et al. Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signatures. Nat Commun 13:3147 (2022). PubMed: 35672317
- Tan JP et al. Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts. Nat Protoc 17:2739-2759 (2022). PubMed: 36241724
- Qiu W et al. RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion. Front Cell Dev Biol 9:617172 (2021). PubMed: 34350169
- Shahbazi MN et al. Developmental potential of aneuploid human embryos cultured beyond implantation. Nat Commun 11:3987 (2020). PubMed: 32778678