Anti-HLA G antibody [MEM-G/9] (Allophycocyanin) (ab40915)

Overview

  • Product nameAnti-HLA G antibody [MEM-G/9] (Allophycocyanin)
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/9] to HLA G (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • SpecificityThis antibody is specific for the native form of HLA G and the soluble HLA G5 isoform in its beta2 microglobulin associated form. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human HLA G protein refolded with beta2 microglobulin and peptide.

  • General notes

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe conjugate is purified by size exclusion chromatography then adjusted for direct use.
  • ClonalityMonoclonal
  • Clone numberMEM-G/9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificityExpressed in trophoblasts.
  • Sequence similaritiesBelongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [MEM-G/9] (Allophycocyanin) images

  • Double surface staining of HLA-G1 transfectants (viable cells gate).

    ab40915 was used at a recommended concentration of 1-5 μg/mL.

References for Anti-HLA G antibody [MEM-G/9] (Allophycocyanin) (ab40915)

This product has been referenced in:
  • Zhao L  et al. Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens 80:231-8 (2012). Read more (PubMed: 22738368) »

See 1 Publication for this product

Product Wall

A suitable Isotype Control to use in Flow Cytometry with ab40915 antibody is http://www.abcam.com/Mouse-IgG1-1F8-Allophycocyanin-Isotype-Control-ab91358.html, as it matches the primary antibody’s host species, isotype, and conjugation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"