Overview

  • Product nameAnti-HLA G antibody [MEM-G/9] (FITC)
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/9] to HLA G (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityReacts with native form of human HLA-G on the cell surface. Specific reaction was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Non Human Primates
  • Immunogen

    Recombinant human HLA G refolded with beta 2 microglobulin and peptide.

  • Positive control
    • JEG-3 human choriocarcinoma cell line
  • General notesAvoid prolonged exposure to light.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThe antibody was purified from ascites by precipitation methods (purity > 95 % by PAGE) and conjugated with fluorescein isothiocyanate (FITC). Excess of FITC was removed by dialysis against PBS containing 15mM sodium azide.
  • ClonalityMonoclonal
  • Clone numberMEM-G/9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 20713594
Flow Cyt 1/500. Do not fix cells (see Menier et al).



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificityExpressed in trophoblasts.
  • Sequence similaritiesBelongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

References for Anti-HLA G antibody [MEM-G/9] (FITC) (ab7904)

This product has been referenced in:
  • La Manna G  et al. Neutrophil gelatinase-associated lipocalin increases HLA-G(+)/FoxP3(+) T-regulatory cell population in an in vitro model of PBMC. PLoS One 9:e89497 (2014). Flow Cyt ; Human . Read more (PubMed: 24586826) »
  • Zhao L  et al. Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens 80:231-8 (2012). Read more (PubMed: 22738368) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us.

As discussed yesterday please buy ab18435 as isotype control.

The clone has been mentioned in many publications, most of the time the cells have been permeabilized so I would suggest permeabilizing the ...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

Read More

Thank you foryoru enquiry.

Regret to inform you that we have no experiences with intracellular staining of ab7904: HLA-G. We have not tested this and we do not have a proved protocol for this. In general, when we need to perform intracellular...

Read More

I would suggest that you should carry out a preliminary experiment to test different dilutions of the primary antibody (i.e. 0.1-10 microgram/ml) and then adjust the dilution accordingly to the results obtained.

As this antibody is conjugated to FITC we do not recommend that you freeze the antibody. Store at 2-8 deg C in the dark - it will be stable for much longer than 1 month.

This antibody is currently in stock, so we can dispatch it within 24 hours of receiving your order (unless we receive it on a Friday, in which case we will dispatch on Monday to avoid weekend delivery). The antibody is stable for at least 1 month at 4 ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"