Overview

  • Product name
  • Description
    Goat polyclonal to HLTF
  • Specificity
    This antibody is expected to recognise both human isoforms of this protein.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-KQAKINEIRTLIDL

    , corresponding to C terminal amino acids 996-1009 of Human HLTF(Peptide available as ab22937.)

  • Positive control
    • Jurkat lysate.
  • General notes
    Principal Names – SMARCA3; HLTF; ZBU1; HLTF1; HIP116; SNF2L3; HIP116A; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SNF2-like 3; sucrose nonfermenting-like 3; helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor-1 regulator. Official Gene Symbol - SMARCA3. GenBank Accession Number – NP_003062; NP_620636. LocusLink ID - 6596 (human); 20585 (mouse). Gene Ontology terms - adenosinetriphosphatase; DNA binding; regulation of transcription, DNA-dependent; nucleus.

Properties

Applications

Our Abpromise guarantee covers the use of ab2835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western Blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate.
    Recommended for use at 0.2-1.0ug/ml (predicted molecular weight: 114 kDa).

    Please note that the weeker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).
  • Target

    • Function
      Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
    • Tissue specificity
      Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
      • Helicase like transcription factor antibody
      • Helicase-like transcription factor antibody
      • HIP116 antibody
      • HIP116A antibody
      • HLTF 1 antibody
      • Hltf antibody
      • HLTF_HUMAN antibody
      • HLTF1 antibody
      • p113 antibody
      • RING finger protein 80 antibody
      • RNF80 antibody
      • SMARC A3 antibody
      • SMARCA 3 antibody
      • SMARCA3 antibody
      • SNF2-like 3 antibody
      • SNF2L3 antibody
      • Sucrose nonfermenting protein 2 like 3 antibody
      • Sucrose nonfermenting protein 2-like 3 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
      • ZBU 1 antibody
      • ZBU1 antibody
      see all

    Images



    • Predicted band size : 114 kDa


      ab2835 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    ab2835 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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