Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre determined using peptide based assay was 1/312500.

Target

  • FunctionTranscription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.
  • Tissue specificityUbiquitous. Detected in pancreas, brain, spleen, placenta, prostate, thymus, liver, heart, bone marrow, skeletal muscle, stomach, uterus, testis, kidney, ovary, and colon.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus. Cytoplasm. Predominantly detected in cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21616 antibody
    • HMBOX1 antibody
    • HMBOX1A antibody
    • HMBX1_HUMAN antibody
    • HNF1LA antibody
    • homeobox containing 1 antibody
    • Homeobox containing protein 1 antibody
    • Homeobox-containing protein 1 antibody
    • homeobox-containing protein PBHNF antibody
    • PBHNF antibody
    see all

Anti-HMBOX1 antibody images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-HMBOX1 antibody (ab50392) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-HMBOX1 antibody (ab50392)

ab50392 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"