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Rabbit polyclonal to HMBOX1
Predicted to work with:
Recombinant fragment corresponding to a region within amino acids 142-377 of Human HMBOX1 (NP_078843)
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
1/100 - 1/500.
1/100 - 1/200.
Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.
Ubiquitous. Detected in pancreas, brain, spleen, placenta, prostate, thymus, liver, heart, bone marrow, skeletal muscle, stomach, uterus, testis, kidney, ovary, and colon.
Contains 1 homeobox DNA-binding domain.
Nucleus. Cytoplasm. Predominantly detected in cytoplasm.
Information by UniProt
Western blot - Anti-HMBOX1 antibody (ab97643)
Anti-HMBOX1 antibody (ab97643) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 47 kDa 10% SDS-PAGE
Immunocytochemistry/ Immunofluorescence - Anti-HMBOX1 antibody (ab97643)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97643 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMBOX1 antibody (ab97643)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft using ab97643 at 1/500 dilution.
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