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Storage instructions Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
1/100 - 1/500.
1/100 - 1/200.
Function Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.
Tissue specificity Ubiquitous. Detected in pancreas, brain, spleen, placenta, prostate, thymus, liver, heart, bone marrow, skeletal muscle, stomach, uterus, testis, kidney, ovary, and colon.
Sequence similarities Contains 1 homeobox DNA-binding domain.
Cellular localization Nucleus. Cytoplasm. Predominantly detected in cytoplasm.
Information by UniProt
Anti-HMBOX1 antibody images
Western blot - HMBOX1 antibody (ab97643)
Anti-HMBOX1 antibody (ab97643) at 1/1000 dilution + Raji whole cell lysate at 30 µg Predicted band size : 47 kDa
Immunocytochemistry/ Immunofluorescence - HMBOX1 antibody (ab97643)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97643 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMBOX1 antibody (ab97643)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft using ab97643 at 1/500 dilution.
References for Anti-HMBOX1 antibody (ab97643)
This product has been referenced in:
et al. miR-30c-1* promotes natural killer cell cytotoxicity against human hepatoma cells by targeting the transcription factor HMBOX1. Cancer Sci 103:645-52 (2012).
Read more (PubMed: 22320217) »
See 1 Publication
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"