• Product nameAnti-HMBS antibody
    See all HMBS primary antibodies
  • Description
    Mouse monoclonal to HMBS
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-362 of Human HMBS



Our Abpromise guarantee covers the use of ab55166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
    • Tissue specificityIsoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.
    • PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
    • Involvement in diseaseDefects in HMBS are the cause of acute intermittent porphyria (AIP) [MIM:176000]. AIP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AIP is an autosomal dominant form of hepatic porphyria characterized by acute attacks of neurological dysfunctions with abdominal pain, hypertension, tachycardia, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
    • Sequence similaritiesBelongs to the HMBS family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • HEM3_HUMAN antibody
      • HMBS antibody
      • Hydroxymethylbilane synthase antibody
      • PBG D antibody
      • PBG-D antibody
      • PBGD antibody
      • PORC antibody
      • Porphobilinogen deaminase antibody
      • porphyria, acute; Chester type antibody
      • Pre uroporphyrinogen synthase antibody
      • Pre-uroporphyrinogen synthase antibody
      • UPS antibody
      • Uroporphyrinogen I synthase antibody
      • Uroporphyrinogen I synthetase antibody
      see all

    Anti-HMBS antibody images

    • Anti-HMBS antibody (ab55166) at 5 µg/ml + 0.2 µg/lane of full length recombinant HMBS protein with a tag.

      Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

      Predicted band size : 39 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-HMBS antibody (ab55166) at 5 µg/ml

      Lane 1 : 50 µg/lane of transfected 293T cell line lysate
      Lane 2 : 50 µg/lane of non-transfected 293T cell line lysate

      Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 39 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    • ab55166, staining HMBS by Elisa. The detection limit for recombinant tagged HMBS is approximately 1ng/ml as a capture antibody.

    References for Anti-HMBS antibody (ab55166)

    ab55166 has not yet been referenced specifically in any publications.

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