• Product name
  • Description
    Rabbit polyclonal to HMG14
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 50-92 of Human HMG14 (NP_004956.5)

  • Positive control
    • HeLa cell lysate
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.



Our Abpromise guarantee covers the use of ab114127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
    • Sequence similarities
      Belongs to the HMGN family.
    • Post-translational
      Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ27265 antibody
      • FLJ31471 antibody
      • High mobility group (nonhistone chromosomal) protein 14 antibody
      • High mobility group nucleosome binding 1 antibody
      • High mobility group nucleosome binding domain 1 antibody
      • High mobility group nucleosome binding domain containing protein 1 antibody
      • High mobility group nucleosome-binding domain-containing protein 1 antibody
      • High mobility group protein 14 antibody
      • HMG 14 antibody
      • HMG14 antibody
      • HMGN 1 antibody
      • HMGN1 antibody
      • HMGN1_HUMAN antibody
      • MGC104230 antibody
      • MGC117425 antibody
      • Non-histone chromosomal protein HMG-14 antibody
      • Nonhistone chromosomal protein HMG 14 antibody
      • Nonhistone chromosomal protein HMG14 antibody
      see all

    Anti-HMG14 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HMG14 with ab114127 at 1/1000 (1µg/ml). Detection: DAB.
    • ab114127 at 10ug/mg of lysate detecting HMG14 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.

    References for Anti-HMG14 antibody (ab114127)

    ab114127 has not yet been referenced specifically in any publications.

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