Overview

  • Product nameAnti-HMG14 antibody
    See all HMG14 primary antibodies
  • Description
    Rabbit polyclonal to HMG14
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human HMG14 around the phosphorylation site of serine 21.

  • Positive control
    • HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionBinds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similaritiesBelongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27265 antibody
    • FLJ31471 antibody
    • High mobility group (nonhistone chromosomal) protein 14 antibody
    • High mobility group nucleosome binding 1 antibody
    • High mobility group nucleosome binding domain 1 antibody
    • High mobility group nucleosome binding domain containing protein 1 antibody
    • High mobility group nucleosome-binding domain-containing protein 1 antibody
    • High mobility group protein 14 antibody
    • HMG 14 antibody
    • HMG14 antibody
    • HMGN 1 antibody
    • HMGN1 antibody
    • HMGN1_HUMAN antibody
    • MGC104230 antibody
    • MGC117425 antibody
    • Non-histone chromosomal protein HMG-14 antibody
    • Nonhistone chromosomal protein HMG 14 antibody
    • Nonhistone chromosomal protein HMG14 antibody
    see all

Anti-HMG14 antibody images

  • Immunofluorescence analysis of HMG14 expression in HepG2 cells, using 1/500 ab63358. Left: untreaded cells. Right: cells treated with immunising peptide.

References for Anti-HMG14 antibody (ab63358)

ab63358 has not yet been referenced specifically in any publications.

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