Overview

  • Product nameAnti-HMG14 antibody
    See all HMG14 primary antibodies
  • Description
    Rabbit polyclonal to HMG14
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 36-85 (PKKAAAKDKS SDKKVQTKGK RGAKGKQAEV ANQETKEDLP AENGETKTEE) of human HMGN1 (NP_004956).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • FunctionBinds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similaritiesBelongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27265 antibody
    • FLJ31471 antibody
    • High mobility group (nonhistone chromosomal) protein 14 antibody
    • High mobility group nucleosome binding 1 antibody
    • High mobility group nucleosome binding domain 1 antibody
    • High mobility group nucleosome binding domain containing protein 1 antibody
    • High mobility group nucleosome-binding domain-containing protein 1 antibody
    • High mobility group protein 14 antibody
    • HMG 14 antibody
    • HMG14 antibody
    • HMGN 1 antibody
    • HMGN1 antibody
    • HMGN1_HUMAN antibody
    • MGC104230 antibody
    • MGC117425 antibody
    • Non-histone chromosomal protein HMG-14 antibody
    • Nonhistone chromosomal protein HMG 14 antibody
    • Nonhistone chromosomal protein HMG14 antibody
    see all

Anti-HMG14 antibody images

  • Anti-HMG14 antibody (ab83184) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 11 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,41 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMG14 with ab83184 at 5.0µg/ml.

References for Anti-HMG14 antibody (ab83184)

ab83184 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"