Anti-HMG14 antibody - N-terminal (ab171762)

Overview

  • Product name
    Anti-HMG14 antibody - N-terminal
    See all HMG14 primary antibodies
  • Description
    Rabbit polyclonal to HMG14 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HMG14 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P05114

  • Positive control
    • Human skin tissue; 293 cell lysate transiently transfected with HMGN1; HepG2 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab171762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 11 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similarities
    Belongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27265 antibody
    • FLJ31471 antibody
    • High mobility group (nonhistone chromosomal) protein 14 antibody
    • High mobility group nucleosome binding 1 antibody
    • High mobility group nucleosome binding domain 1 antibody
    • High mobility group nucleosome binding domain containing protein 1 antibody
    • High mobility group nucleosome-binding domain-containing protein 1 antibody
    • High mobility group protein 14 antibody
    • HMG 14 antibody
    • HMG14 antibody
    • HMGN 1 antibody
    • HMGN1 antibody
    • HMGN1_HUMAN antibody
    • MGC104230 antibody
    • MGC117425 antibody
    • Non-histone chromosomal protein HMG-14 antibody
    • Nonhistone chromosomal protein HMG 14 antibody
    • Nonhistone chromosomal protein HMG14 antibody
    see all

Images

  • All lanes : Anti-HMG14 antibody - N-terminal (ab171762) at 1/100 dilution

    Lane 1 : 293 cell lysate, non-transfected
    Lane 2 : 293 cell lysate, transiently transfected with the HMGN1 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 11 kDa

  • Anti-HMG14 antibody - N-terminal (ab171762) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size: 11 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling HMG14 with ab171762 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171762 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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