Anti-HMG14 (phospho S20 + S24) antibody (ab115307)

Overview

  • Product nameAnti-HMG14 (phospho S20 + S24) antibody
    See all HMG14 primary antibodies
  • Description
    Rabbit polyclonal to HMG14 (phospho S20 + S24)
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    RRSARLSAKP

    conjugated to KLH, corresponding to amino acids 19-28 of Human HMG14 phosphorylated at Serine 20 and 24.

  • Positive control
    • Human skin tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 11 kDa.
ELISA 1/5000 - 1/25000.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionBinds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similaritiesBelongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27265 antibody
    • FLJ31471 antibody
    • High mobility group (nonhistone chromosomal) protein 14 antibody
    • High mobility group nucleosome binding 1 antibody
    • High mobility group nucleosome binding domain 1 antibody
    • High mobility group nucleosome binding domain containing protein 1 antibody
    • High mobility group nucleosome-binding domain-containing protein 1 antibody
    • High mobility group protein 14 antibody
    • HMG 14 antibody
    • HMG14 antibody
    • HMGN 1 antibody
    • HMGN1 antibody
    • HMGN1_HUMAN antibody
    • MGC104230 antibody
    • MGC117425 antibody
    • Non-histone chromosomal protein HMG-14 antibody
    • Nonhistone chromosomal protein HMG 14 antibody
    • Nonhistone chromosomal protein HMG14 antibody
    see all

Anti-HMG14 (phospho S20 + S24) antibody images

  • ab115307 at 20ug/ml staining HMG14 (phospho S20 + S24) in Human skin tissue (FFPE) by immunohistochemistry. Slides were subject to heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-HMG14 (phospho S20 + S24) antibody (ab115307)

ab115307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"