Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
Belongs to the HMGN family.
Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
ab115307 at 20ug/ml staining HMG14 (phospho S20 + S24) in Human skin tissue (FFPE) by immunohistochemistry. Slides were subject to heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.