Anti-HMG14 (phospho S20 + S24) antibody (ab34760)

Overview

  • Product nameAnti-HMG14 (phospho S20 + S24) antibody
    See all HMG14 primary antibodies
  • Description
    Rabbit polyclonal to HMG14 (phospho S20 + S24)
  • Specificityab34760 recognises human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human HMG14 aa 19-28.
    Sequence:

    RRSARLSAKP

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab34760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/25000.
WB 1/500 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).

Target

  • FunctionBinds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similaritiesBelongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27265 antibody
    • FLJ31471 antibody
    • High mobility group (nonhistone chromosomal) protein 14 antibody
    • High mobility group nucleosome binding 1 antibody
    • High mobility group nucleosome binding domain 1 antibody
    • High mobility group nucleosome binding domain containing protein 1 antibody
    • High mobility group nucleosome-binding domain-containing protein 1 antibody
    • High mobility group protein 14 antibody
    • HMG 14 antibody
    • HMG14 antibody
    • HMGN 1 antibody
    • HMGN1 antibody
    • HMGN1_HUMAN antibody
    • MGC104230 antibody
    • MGC117425 antibody
    • Non-histone chromosomal protein HMG-14 antibody
    • Nonhistone chromosomal protein HMG 14 antibody
    • Nonhistone chromosomal protein HMG14 antibody
    see all

Anti-HMG14 (phospho S20 + S24) antibody images

  • ab34760 at 20 µg/ml staining of epidermal keratinocytes at 40X.  This image shows moderate nuclear and faint cytoplasmic positive staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

References for Anti-HMG14 (phospho S20 + S24) antibody (ab34760)

ab34760 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"