Overview

  • Product nameAnti-HMG20A antibody
    See all HMG20A primary antibodies
  • Description
    Rabbit polyclonal to HMG20A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
  • Immunogen

    A region within synthetic peptide: SMPLPGSGET PTVDTIDSYM NRLHSIILAN PQDNENFIAT VREVVNRLDR, corresponding to amino acids 298-347 of Human HMG20A

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

Anti-HMG20A antibody images

  • Anti-HMG20A antibody (ab50590) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-HMG20A antibody (ab50590)

ab50590 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"