Overview

  • Product name
  • Description
    Rabbit polyclonal to HMG20A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ENLMTSSTLP PLFADEDGSK ESNDLATTGL NHPEVPYSSG ATSSTNNPEF) of human HMG20A (NP_060670).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

Target

  • Function
    Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10739 antibody
    • High mobility group 20A antibody
    • High mobility group protein 20A antibody
    • HM20A_HUMAN antibody
    • HMG box containing protein 20A antibody
    • HMG box domain containing 1 antibody
    • HMG box-containing protein 20A antibody
    • HMG domain protein HMGX1 antibody
    • HMG domain-containing protein 1 antibody
    • HMG domain-containing protein HMGX1 antibody
    • hmg20a antibody
    • HMGX1 antibody
    • HMGXB1 antibody
    • Hypothetical protein FLJ10739 antibody
    • iBRAF antibody
    • Inhibitor of BRAF35 antibody
    see all

Images

  • Anti-HMG20A antibody (ab83284) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

References

ab83284 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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