Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 0.3 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBinds preferentially single-stranded DNA and unwinds double stranded DNA.
  • Tissue specificityExpressed predominantly in placenta.
  • Sequence similaritiesBelongs to the HMGB family.
    Contains 2 HMG box DNA-binding domains.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • chromosomal protein, Nonhistone, HMG4 antibody
    • High mobility group (nonhistone chromosomal) protein 4 antibody
    • High mobility group box 3 antibody
    • High mobility group protein 2a antibody
    • High mobility group protein 4 antibody
    • High mobility group protein B3 antibody
    • High mobility group protein HMG4 antibody
    • HMG 4 antibody
    • HMG-2a antibody
    • HMG-4 antibody
    • HMG2A antibody
    • HMGB 3 antibody
    • Hmgb3 antibody
    • HMGB3_HUMAN antibody
    • MGC90319 antibody
    • Non histone chromosomal protein antibody
    • Nonhistone chromosomal protein HMG4 antibody
    see all

Anti-HMG4 antibody images

  • Anti-HMG4 antibody (ab28727) at 0.3 µg/ml + Placenta tissue lysate

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 23 kDa
  • Ab28727, at a concentration of 4-8 µg/ml, staining HMG4 in paraffin embedded human intestine sections by Immunohistochemistry. Positively labelled epithelial cells of intestinal villus are indicated by arrows.

References for Anti-HMG4 antibody (ab28727)

ab28727 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (hela cells)
Loading amount 30 µg
Specification hela cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
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Verified customer

Submitted Mar 08 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"