Overview

  • Product name
    Anti-HMG4 antibody [EP2839Y]
    See all HMG4 primary antibodies
  • Description
    Rabbit monoclonal [EP2839Y] to HMG4
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human HMG4

  • Positive control
    • 293T, HeLa and human placenta cell lysates, human placenta tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Binds preferentially single-stranded DNA and unwinds double stranded DNA.
    • Tissue specificity
      Expressed predominantly in placenta.
    • Sequence similarities
      Belongs to the HMGB family.
      Contains 2 HMG box DNA-binding domains.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • chromosomal protein, Nonhistone, HMG4 antibody
      • High mobility group (nonhistone chromosomal) protein 4 antibody
      • High mobility group box 3 antibody
      • High mobility group protein 2a antibody
      • High mobility group protein 4 antibody
      • High mobility group protein B3 antibody
      • High mobility group protein HMG4 antibody
      • HMG 4 antibody
      • HMG-2a antibody
      • HMG-4 antibody
      • HMG2A antibody
      • HMGB 3 antibody
      • Hmgb3 antibody
      • HMGB3_HUMAN antibody
      • MGC90319 antibody
      • Non histone chromosomal protein antibody
      • Nonhistone chromosomal protein HMG4 antibody
      see all

    Images

    • All lanes : Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : human placenta cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible this band results from an immunoprecipitated sample, it is the ~55kDa heavy chain of the antibody used for the IP).
    • Immunohistochemical analysis of paraffin-embedded human placenta using ab75782 at 1/100-1/250 dilution and with the antigen retrieval step.

    References

    ab75782 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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