Overview

  • Product nameAnti-HMGA1a / HMGA1b antibody
    See all HMGA1a / HMGA1b primary antibodies
  • Description
    Goat polyclonal to HMGA1a / HMGA1b
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-SQPLASKQEKDGT,

    corresponding to internal sequence amino acids 9-21 of Human HMGA1a/ HMGA1b (NP_665906.1; NP_002122.1).

  • Positive control
    • WB: pancreas lysate. IHC-P: placenta or skin tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • FunctionHMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  • Involvement in diseaseNote=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
  • Sequence similaritiesBelongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Post-translational
    modifications
    Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
    HMG-Y is not methylated.
    Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • High mobility group AT hook 1 antibody
    • High mobility group AT-hook protein 1 antibody
    • High mobility group protein A1 antibody
    • High mobility group protein HMG-I/HMG-Y antibody
    • High mobility group protein R antibody
    • HMG-I(Y) antibody
    • HMGA 1a antibody
    • HMGA 1b antibody
    • HMGA1 antibody
    • HMGA1_HUMAN antibody
    • HMGI antibody
    • HMGI/Y antibody
    • HMGR antibody
    • HMGY antibody
    see all

Anti-HMGA1a / HMGA1b antibody images

  • Anti-HMGA1a / HMGA1b antibody (ab109706) at 0.5 µg/ml (incubation time: 1 hour) + Human pancreas lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 12 kDa
  • ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human placenta tissue.
  • ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human skin tissue.

References for Anti-HMGA1a / HMGA1b antibody (ab109706)

ab109706 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"