Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).
Our Abpromise guarantee covers the use of ab97276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 12 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of an anonymous abreview.