Overview

  • Product nameAnti-HMGA2 antibody
    See all HMGA2 primary antibodies
  • Description
    Rabbit polyclonal to HMGA2
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).

  • Positive control
    • MOLT4 whole cell lysate, MCF7 cells, HepG2, HCT116, HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab97276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionFunctions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • Involvement in diseaseNote=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • Sequence similaritiesBelongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Developmental stageExpressed predominantly during embryogenesis.
  • Post-translational
    modifications
    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Anti-HMGA2 antibody images

  • Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 12 kDa
  • ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HCT116

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 12 kDa

References for Anti-HMGA2 antibody (ab97276)

This product has been referenced in:
  • Meijer HA  et al. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340:82-5 (2013). WB . Read more (PubMed: 23559250) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Primary Fetal Fibroblast)
Gel Running Conditions Reduced Denaturing (Mini-PROTEAN® TGX™ 4-20%)
Loading amount 50 µg
Specification Primary Fetal Fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Nov 21 2016

Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Negative control DW No-antibody
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control input (whole lysate)
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Submitted Sep 19 2014

The predicted size of HMGA2 is at 12 kDa, but it is also reported to migrate at higher size around 17-19 kDa probably due to PTM . (see publication: http://www.ncbi.nlm.nih.gov/pubmed/15026339)

The Uniprot database also shows HMGA2 has severa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA)
Loading amount 25 µg
Specification HeLA
Gel Running Conditions Reduced Denaturing (17.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"