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Products:Epigenetics and Nuclear Signaling >> Histones >> HMGs
Overview
Product name
Anti-HMGB1 antibody [HAP46.5]
See all HMGB1 products (14) ...
Description
Mouse monoclonal [HAP46.5] to HMGB1
Specificity
The alignment of the immunogen for ab12029 and HMBG2 is high at 81%. Therefore, it is possible this antibody may also detect HMGB2.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Dog, Human
Immunogen
Recombinant full length protein (Human).
Positive control
Total cell extract of human MCF breast carcinoma cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
HAP46.5
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ELISA: Use at an assay dependent dilution.
Application notes
Is unsuitable for or ICC/IF.
Target
Function
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP09429
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amphoterin antibody
- DKFZp686A04236 antibody
- High mobility group 1 antibody
see all
Database links
- Entrez Gene: 3146 Human
- Entrez Gene: 15289 Mouse
- Entrez Gene: 25459 Rat
- Omim: 163905 Human
- SwissProt: P36194 Chicken
- SwissProt: P09429 Human
- SwissProt: P07155 Mouse
- SwissProt: P63158 Mouse
see all
References for Anti-HMGB1 antibody [HAP46.5] (ab12029)
This product has been referenced in:
- Su Xet al. Requisite role of the cholinergic alpha7 nicotinic acetylcholine receptor pathway in suppressing Gram-negative sepsis-induced acute lung inflammatory injury. J Immunol 184:401-10 (2010). ELISA; Mouse.Read more (PubMed: 19949071) »
- Bitto Aet al. High mobility group box-1 expression correlates with poor outcome in lung injury patients. Pharmacol Res 61:116-20 (2010). WB; Human.Read more (PubMed: 19666121) »
See all 7 publications for this product
Publishing research using ab12029? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"