Anti-HMGB2 antibody (ab67282)
Key features and details
- Rabbit polyclonal to HMGB2
- Suitable for: ICC, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HMGB2 antibody
See all HMGB2 primary antibodies -
Description
Rabbit polyclonal to HMGB2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Opossum -
Immunogen
Synthetic peptide:
KGKSEAGKKGPGRPTGS
conjugated to KLH, corresponding to amino acids 165-181 of Human HMGB2 -
Positive control
- WB: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
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IP |
Use at an assay dependent concentration. Use 5-10µg.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
IP
Use at an assay dependent concentration. Use 5-10µg. |
Target
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Function
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). -
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 540444 Cow
- Entrez Gene: 3148 Human
- Entrez Gene: 97165 Mouse
- Entrez Gene: 397130 Pig
- Entrez Gene: 29395 Rat
- Entrez Gene: 498072 Rat
- Omim: 163906 Human
- SwissProt: P40673 Cow
see all -
Alternative names
- C80539 antibody
- High mobility group (nonhistone chromosomal) protein 2 antibody
- High mobility group box 2 antibody
see all
Images
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Immunocytochemistry analysis of HUVEC cells using Anti-HMGB2 antibody (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).
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All lanes : Anti-HMGB2 antibody (ab67282) at 2 mg/ml
Lane 1 : NIH3T3
Lane 2 : Rat1
Lane 3 : RINSH
Lane 4 : PC12
Lane 5 : K562
Lane 6 : HEK-293T
Lane 7 : HeLa nuclear
Lane 8 : HeLa
Predicted band size: 24 kDa -
Nuclear localization of HMGB2 in PC12 cells.
A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 µg/mL
B. DAPI staining
C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2 -
Lanes
1: 5 µg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
2: Negative control – without cell lysate
3: Negative control – without antibody
4: Positive control – Extract of HEK-293T direct labeling
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab67282 has been referenced in 30 publications.
- Sato M et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun 12:2529 (2021). PubMed: 33953175
- Sofiadis K et al. HMGB1 coordinates SASP-related chromatin folding and RNA homeostasis on the path to senescence. Mol Syst Biol 17:e9760 (2021). PubMed: 34166567
- Wang W et al. LINC00184 plays an oncogenic role in non-small cell lung cancer via regulation of the miR-524-5p/HMGB2 axis. J Cell Mol Med 25:9927-9938 (2021). PubMed: 34651416
- Tan HY et al. Downregulation of miR-223 promotes HMGB2 expression and induces oxidative stress to activate JNK and promote autophagy in an in vitro model of acute lung injury. J Inflamm (Lond) 18:29 (2021). PubMed: 34732212
- Li J et al. Single-cell RNA Sequencing Reveals Thoracolumbar Vertebra Heterogeneity and Rib-genesis in Pigs. Genomics Proteomics Bioinformatics 19:423-436 (2021). PubMed: 34775075