Our Abpromise guarantee covers the use of ab67282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 21890638Use 5µg|
|CHIPseq||Use at an assay dependent concentration. PubMed: 27226577|
|IHC-P||Use at an assay dependent concentration. PubMed: 27226577|
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 24 kDa.|
|IP||Use at an assay dependent concentration. Use 5-10µg.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
Cross-linking chIP was performed using HMGB2 antibody (ab67282) and 25million HUVECs. The cells were crosslinked with 15mM ethylene glycol bis(succinimidylsuccinate) (EGS) for 20 minutes at room temperature, followed by the addition of Complete Cell Fixative Solution for 40 minutes at 4°C. 4µg of HMGB2 antibody was added per 25µg of sheared chromatin. qPCR was performed using positive primers against the 5’ prime UTR OF ZNF335 and HIST2H2AC genes and negative primer against the olfactory region 8.