Overview

  • Product nameAnti-HMGB2 antibody - ChIP Grade
    See all HMGB2 primary antibodies
  • Description
    Rabbit polyclonal to HMGB2 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide:

    KGKSEAGKKGPGRPTGS

    conjugated to KLH, corresponding to amino acids 165-181 of Human HMGB2

  • Positive control
    • PC12 whole cell lysate. HEK-293T cell lysate. PC12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21890638Use 5µg
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 24 kDa.
IP Use at an assay dependent dilution. Use 5-10µg.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

Anti-HMGB2 antibody - ChIP Grade images

  • All lanes : Anti-HMGB2 antibody - ChIP Grade (ab67282) at 2 mg/ml

    Lane 1 : NIH3T3
    Lane 2 : Rat1
    Lane 3 : RINSH
    Lane 4 : PC12
    Lane 5 : K562
    Lane 6 : HEK-293T
    Lane 7 : HeLa nuclear
    Lane 8 : HeLa


    Predicted band size : 24 kDa
  • Nuclear localization of HMGB2 in PC12 cells.
    A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 μg/mL
    B. DAPI staining
    C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2
  • Lanes
    1: 5 μg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
    2: Negative control – without cell lysate
    3: Negative control – without antibody
    4: Positive control – Extract of HEK-293T direct labeling

References for Anti-HMGB2 antibody - ChIP Grade (ab67282)

This product has been referenced in:
  • Tanco S  et al. C-terminomics screen for natural substrates of cytosolic carboxypeptidase 1 reveals processing of acidic protein C termini. Mol Cell Proteomics 14:177-90 (2015). Read more (PubMed: 25381060) »
  • Redmond AM  et al. Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells. Oncogene N/A:N/A (2014). WB, IP ; Human . Read more (PubMed: 25284587) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (HUVEC)
Negative control olfactory region 8
Specification HUVEC
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: 15mM EGS 20 min at R/T. additi
Positive control 5’ prime UTR OF ZNF335 and HIST2H2AC genes
Username

Dr. Theodore Georgomanolis

Verified customer

Submitted May 31 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (skeletal muscle)
Permeabilization Yes - 0.1% Triton X100
Specification skeletal muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 05 2015

Thank you for your reply. I can confirm that the immunogen for ab18256 HMGB1 antibody (KPDAAKKGVVKAEKSK) has 43% alignment to the HMGB2 protein.  This is low,  so the ab18256 HMGB1 antibody is not likely to detect HMGB2. The immunogen for ab67282...

Read More

Thank you for your enquiry. The immunogen for ab67282 HMGB2 antibody has very low alignment to the HMGB1 protein, so is not likely to detect HMGB1.  However, this has not been specifically tested in the laboratory. The ab12029 HMGB1 immunogen d...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"