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Product name Anti-HMGB2 antibody [EPR6301] See all HMGB2 primary antibodies
Rabbit monoclonal [EPR6301] to HMGB2
Tested applications Suitable for:
ICC/IF, WB, IP, IHC-P more details
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HMGB2 (N terminal). The exact sequence is proprietary.
WB: K562, HeLa and PC12 cell lysates.
ICC/IF: PC-12 cells.
IHC-P: Human breast tissue.
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Anti-HMGB2 antibody (Alexa Fluor® 647) [EPR6301] ( ab207368) Anti-HMGB2 antibody (Alexa Fluor[EPR6301] ( ® 488) ab208388)
Produced using Abcam's RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K D)
K D = 6.03 x 10
Learn more about K D
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR6301
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).
Sequence similarities Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains.
Cellular localization Nucleus. Chromosome.
Information by UniProt
High mobility group (nonhistone chromosomal) protein 2 antibody
High mobility group box 2 antibody
Anti-HMGB2 antibody [EPR6301] images
Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)
All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution Lane 1 : K562 cell lysates Lane 2 : HeLa cell lysates Lane 3 : PC12 cell lysates Lysates/proteins at 10 µg per lane. Secondary Goat-anti-rabbit HRP at 1/2000 dilution Predicted band size : 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HMGB2 antibody [EPR6301] (ab124670)
Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
ab150077, an Alexa Fluor 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. ® Control: PBS only. Nuclear counter stain: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301] (ab124670)
ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
Other-Anti-HMGB2 antibody [EPR6301](ab124670)
References for Anti-HMGB2 antibody [EPR6301] (ab124670)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"