Anti-HMGB2 antibody [EPR6301] (ab124670)

Overview

  • Product nameAnti-HMGB2 antibody [EPR6301]
    See all HMGB2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6301] to HMGB2
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HMGB2 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: K562, HeLa and PC12 cell lysates. ICC/IF: PC-12 cells. IHC-P: Human breast tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-HMGB2 antibody (Alexa Fluor® 647) [EPR6301] (ab207368)
    Anti-HMGB2 antibody (Alexa Fluor® 488) [EPR6301] (ab208388)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 6.03 x 10 -10 M Learn more about KD
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6301
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

Anti-HMGB2 antibody [EPR6301] images

  • All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : PC12 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 24 kDa
  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-HMGB2 antibody [EPR6301] (ab124670)

ab124670 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Sample Human Cell lysate - nuclear (HUVEC)
Negative control olfactory region 8 (OR8)
Specification HUVEC
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: 20 min EGS + 40 min with PFA w
Positive control 5’ prime UTR of ZNF335 and HIST2H2AC genes
Username

Dr. Theodore Georgomanolis

Verified customer

Submitted May 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"