Overview

  • Product name
  • Description
    Rabbit polyclonal to HMGB4
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HMGB4 aa 24-41 (N terminal). The human HMGB4 is different to the related mouse and rat sequence by a single amino acid.
    Sequence:

    RNKFKEQQPNTYVGFKEF


    Database link: Q8WW32

  • Positive control
    • Rat testicle tissue lysis

Properties

Applications

Our Abpromise guarantee covers the use of ab65980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC Use at an assay dependent dilution.

Target

  • Relevance
    High Mobility Group (HMG) proteins, a group of chromosomal proteins common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation, helping with transcription, replication, recombination, and DNA repair. HMGB4 is HMG2-like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, and is found in a variety of eukaryotic chromosomal proteins. HMGB proteins are phosphorylated to various extents. The existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • High mobility group box 4 antibody
    • High mobility group protein B4 antibody
    • HMG2 like antibody

Images

  • All lanes : Anti-HMGB4 antibody (ab65980) at 1/2 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat testicular tissue lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : HT1080 whole cell lysate


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • Anti-HMGB4 antibody (ab65980) at 1 µg/ml + rat testicle tissue lysis at 30 µg

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

References

This product has been referenced in:
  • Zhang Z  et al. HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/ß-catenin pathway. PLoS One 12:e0179741 (2017). WB ; Human . Read more (PubMed: 28678825) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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