Overview

  • Product name
  • Description
    Rabbit polyclonal to HMGCS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 194-455 of Human HMGCS1 (Uniprot ID: Q01581).

  • Positive control
    • 293T, A431, HeLa and Mouse liver whole cell lysates; Hela cells; Rat RT2 xenograft and Human CL15 xenograft tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab155787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-HMGCS1 antibody (ab155787) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Anti-HMGCS1 antibody (ab155787) at 1/1000 dilution + Mouse liver whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling HMGCS1 with ab155787 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling HMGCS1 with ab155787 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human CL15 xenograft tissue, labeling HMGCS1 with ab155787 at 1/500 dilution.

References

This product has been referenced in:
  • Szántó M  et al. Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL levels. Biochim Biophys Acta 1842:594-602 (2014). Mouse . Read more (PubMed: 24365238) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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