This antibody gave a positive signal in Human Liver tissue lysate.
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1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney.
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Involvement in disease
Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids.
3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
HMG CoA synthase antibody
HMG-CoA synthase antibody
HMGCS 2 antibody
Hydroxymethylglutaryl CoA synthase antibody
Hydroxymethylglutaryl CoA synthase mitochondrial antibody
Hydroxymethylglutaryl-CoA synthase antibody
Western blot - Anti-HMGCS2 antibody (ab157225)
Anti-HMGCS2 antibody (ab157225) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa Observed band size: 56 kDa Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab157225 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.