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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity Tissue culture supernatant
Clone number EPR7091
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/100 - 1/250.
Application notes Is unsuitable for Flow Cyt or IP.
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
Sequence similarities Belongs to the HMGN family.
Post-translational modifications Phosphorylation favors cytoplasmic localization.
Cellular localization Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
Information by UniProt
High mobility group (nonhistone chromosomal) protein 17 antibody
high mobility group nucleosomal binding domain 2 antibody
High mobility group nucleosome-binding domain-containing protein 2 antibody
Anti-HMGN2 antibody [EPR7091] images
Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution Lane 1 : HL60 cell lysate Lane 2 : K562 cell lysate Lane 3 : HeLa cell lysate Lane 4 : SH-SY5Y cell lysate Lane 5 : Jurkat cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size : 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997)
ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
References for Anti-HMGN2 antibody [EPR7091] (ab124997)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"