1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
Belongs to the HMGN family.
Phosphorylation favors cytoplasmic localization.
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.