• Product nameAnti-HMW Cytokeratin antibody [34bE12]
    See all HMW Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [34bE12] to HMW Cytokeratin
  • SpecificityThis antibody recognizes the HMW (High Molecular Weight) keratin polypeptides of 68, 58, 56.5 and 50 kDa in extract of stratum corneum. The antibody reacts with squamous, ductal and other complex epithelia. It stains adenocarcinomas, breast, pancreas, bile duct, salivary gland and transitional cell carcinomas.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Solubilized keratin extract from human stratum corneum.



Our Abpromise guarantee covers the use of ab776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining.
  • Target

    • RelevanceCytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • CK13 antibody
      • CK14 antibody
      • CK15 antibody
      • CK16 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin 14 antibody
      • Cytokeratin 15 antibody
      • Cytokeratin 16 antibody
      • K13 antibody
      • K14 antibody
      • K15 antibody
      • K16 antibody
      • Keratin 13 antibody
      • Keratin 14 antibody
      • Keratin 15 antibody
      • Keratin 15, basic antibody
      • Keratin 15, beta antibody
      • Keratin 16 antibody
      • KRT13 antibody
      • KRT14 antibody
      • KRT15 antibody
      • KRT16 antibody
      • KRT16A antibody
      see all

    Anti-HMW Cytokeratin antibody [34bE12] images

    • ab776 - immunohistochemistry

      Formalin fixed paraffin embedded human colon carcinoma stained with HMW Cytokeratin, using ABC and AEC chromagen.

    • ab776 staining human skin tissue sections by IHC-P.

    References for Anti-HMW Cytokeratin antibody [34bE12] (ab776)

    This product has been referenced in:
    • Hagman Z  et al. miR-205 negatively regulates the androgen receptor and is associated with adverse outcome of prostate cancer patients. Br J Cancer 108:1668-76 (2013). IHC ; Human . Read more (PubMed: 23571738) »

    See 1 Publication for this product

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