Overview

  • Product nameAnti-HMW Cytokeratin antibody [AE3]
    See all HMW Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE3] to HMW Cytokeratin
  • Specificityab80823 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily.
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Monkey
  • Immunogen

    Human epidermal Keratin

  • Positive control
    • Skin. Lung carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/100 - 1/200 for 20 - 30 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37°C or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-HMW Cytokeratin antibody [AE3] images

    • Formalin-fixed, paraffin-embedded human skin stained with ab80823 at 1/100 dilution using a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.

    References for Anti-HMW Cytokeratin antibody [AE3] (ab80823)

    This product has been referenced in:
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »
    • Cooper D  et al. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation 28:30-5 (1984). Read more (PubMed: 6083891) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"