Anti-HMW Kininogen antibody [C11C1] (ab1004)


  • Product nameAnti-HMW Kininogen antibody [C11C1]
    See all HMW Kininogen primary antibodies
  • Description
    Mouse monoclonal [C11C1] to HMW Kininogen
  • SpecificityThis antibody reacts with the unique light chain (mw~46,000) of human HMWK.
  • Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified high molecular weight kininogen (HMWK).



Our Abpromise guarantee covers the use of ab1004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system.
ELISA Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. This antibody can completely neutralize the coagulant activity of HMWK.


  • Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similaritiesContains 3 cystatin domains.
  • Post-translational
    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 2 thiol proteinase inhibitor antibody
    • Alpha-2-thiol proteinase inhibitor antibody
    • BDK antibody
    • BK antibody
    • Bradykinin antibody
    • Fitzgerald factor antibody
    • High molecular weight kininogen antibody
    • HMWK antibody
    • Ile-Ser-Bradykinin antibody
    • Kallidin I antibody
    • Kallidin II antibody
    • Kininogen 1 antibody
    • KNG antibody
    • KNG1 antibody
    • KNG1_HUMAN antibody
    • Low molecular weight growth-promoting factor antibody
    • Williams-Fitzgerald-Flaujeac factor antibody
    see all

Anti-HMW Kininogen antibody [C11C1] images

  • Image courtesy of an anonymous Abreview.

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References for Anti-HMW Kininogen antibody [C11C1] (ab1004)

This product has been referenced in:
  • Langhauser F  et al. Kininogen deficiency protects from ischemic neurodegeneration in mice by reducing thrombosis, blood-brain barrier damage, and inflammation. Blood 120:4082-92 (2012). WB . Read more (PubMed: 22936662) »
  • Schmaier AH  et al. Determination of the bifunctional properties of high molecular weight kininogen by studies with monoclonal antibodies directed to each of its chains. J Biol Chem 262:1405-11 (1987). Read more (PubMed: 2433279) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cell lines Daudi, Raji, Jurkat, HUVEC,T47D)
Loading amount 20 µg
Specification Cell lines Daudi, Raji, Jurkat, HUVEC,T47D
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Elena Kashuba

Verified customer

Submitted Apr 10 2013

Thank you for your telephone call and I am sorry you have also experienced difficulty with the third vial of ab1004 from this order.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for taking the time to contact us by phone this morniing. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. As discussed on the telephone, we will happily offer technical support and in the even...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Daudi cells,Raji sells,Jurkat cells)
Loading amount 20 µg
Specification Daudi cells,Raji sells,Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 24 2011

Thank you for your enquiry. I cannot recommend one specific antibody over the other. In both cases the difference is that they are different clones. The antibodies work in the same applications but the clone is different. These antibodies will re...

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