Recombinant Anti-HN1 antibody [EPR7364] (ab126705)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7364] to HN1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HN1 antibody [EPR7364]
See all HN1 primary antibodies -
Description
Rabbit monoclonal [EPR7364] to HN1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human HN1 aa 100-200. The exact sequence is proprietary.
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Positive control
- HepG2, Human testis, HeLa, Human fetal muscle, and Human thymus lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7364 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa). |
Target
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Tissue specificity
Expressed in testis, skeletal muscle, thymus, prostate, colon, peripheral blood cells, brain and placenta. -
Sequence similarities
Belongs to the HN1 family. -
Post-translational
modificationsIsoform 3 initiator methionine is either acetylated or removed. In the latter case, the new N-terminal amino acid is then N-acetylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51155 Human
- Entrez Gene: 15374 Mouse
- Entrez Gene: 287828 Rat
- SwissProt: Q9UK76 Human
- SwissProt: P97825 Mouse
- SwissProt: Q6AXU6 Rat
- Unigene: 532803 Human
- Unigene: 1775 Mouse
see all -
Alternative names
- Androgen regulated protein 2 antibody
- Androgen-regulated protein 2 antibody
- ARM2 antibody
see all
Images
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All lanes : Anti-HN1 antibody [EPR7364] (ab126705) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human testis lysate
Lane 4 : Human fetal muscle lysate
Lane 5 : Human thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
ab126705 at 1/10 dilution staining HN1 in permeabilized HeLa cells by intracellular flow cytometry (red) compared to a rabbit IgG negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab126705 has been referenced in 4 publications.
- Wang R et al. The HN1/HMGB1 axis promotes the proliferation and metastasis of hepatocellular carcinoma and attenuates the chemosensitivity to oxaliplatin. FEBS J 289:6400-6419 (2022). PubMed: 35596723
- Tan S et al. Hesperidin administration suppresses the proliferation of lung cancer cells by promoting apoptosis via targeting the miR-132/ZEB2 signalling pathway. Int J Mol Med 46:2069-2077 (2020). PubMed: 33125117
- Pu J et al. hsa_circ_0000092 promotes hepatocellular carcinoma progression through up-regulating HN1 expression by binding to microRNA-338-3p. J Cell Mol Med N/A:N/A (2020). PubMed: 32077624
- Jia Q et al. HNRNPA1-mediated 3' UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes. Aging (Albany NY) 11:4407-4437 (2019). PubMed: 31257225