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ab10685 has been referenced in 9 publications.
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All lanes : Anti-hnRNP A1 antibody [4B10] (ab10685)
Lane 1 : 20ug Jurkat cell lysate
Lane 2 : 20ug Jurkat cell lysate from cells transfected with hnRNP A1 siRNA
Predicted band size : 38.4 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
ab10685 used at a 1/100 dilution in Immunprecipitation. Whole cell lysate prepared from HeLaS3 cells used at 5 x 106 cells. Immunoprecipitation step performed using Dynabeads Protein G. Primary antibody incubated for 3 hours at 4°C. ab10685 used at a 1/2000 dilution in Western Blot.
This image is courtesy of an anonymous abreview.
Anti-hnRNP A1 antibody [4B10] (ab10685) at 1/1000 dilution
Secondary
Anti-Mouse IgG AP Conjugate at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 38.4 kDa
Overlay histogram showing HeLa cells stained with ab10685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10685, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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