Anti-hnRNP A1 antibody [4B10] (ab10685)

Merci de votre réponse.
En regardant la liste des anti-hnRNP A1, notre choix, pour un
remplacement, s'est arrêté sur la référence: anti-hnRNP A1 antibody
[9H10] (ab5832).
Lors des tests, nous utilisons des HeLa nuclear extract

ANSWER:

Merci pour votre réponse.

Désirez-vous recevoir 2 unités d'anti-hnRNP A1 [9H10] (ab5832) en remplacement des 2 lots deab10685 que vous avez reçus?

Je peux également éditer un avoir/remboursement sur la dernière commande et envoyer une seule unité de ab5832 enremplacement du lot commandé en mai 2011.

Merci de m'indiquer votre préférence.

Nous avons commandé en mai 2011 chez vous l'anticorps anti-hnRNP A1 [4B10], lot GR 14303-4, qui n'a pas fonctionné. Nous avons refait unecommande en mars 2012, lot GR 14303-7. Ce nouveau lot a partiellement fonctionné.
Nous avons utilisé cet anticorps aux conditions suivantes lors des mises au point: dilution 1:250; 1:500; 1:1000 lait 5%.
Que nous conseillez-vous de faire?

ANSWER:

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que les 2 lots de ab10685 que vous avez reçus ne fonctionnent pas comme attendu en Western Blot.

Afin de remédier au plus vite à ce problème, je serai ravi de vous envoyer un remplacement par une autre référence parmi la liste des anti-hnRNP A1 suivante (hormis ab10685 bien sur): http://www.abcam.com/index.html?pageconfig=searchresults&search=hnRNP A1&pt=1&sk=targ&sv=hnRNP A1&sn=hnRNP A1&l=1&fViewMore=1



Si un remplacement ne vous convient pas je peux également éditer un avoir.

Afin d'étudier le problème rencontré, pourriez-vous m'indiquer le type d'échantillon que vous étudier (espèce, tissu, lignée cellulaire) ?

Merci de m'indiquer la manière dont vous désirez procéder.

Dear Tech Support Team,

Our customer is willing to test ab5832 or ab10685 in IP with Drosophila samples. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's

website and then we give 100% credit note for this antibody to buy next antibody)?

Do you have information about the homology of the immunogens of these 2 antibodies with Drosophila protein? Which of these two is preferable for the test withthe abreview promotion?

Thanks in advance for your assistance and reply.

ANSWER:

I have not been able to find the Drosophila sequence to check homology of the immunogen for either of these antibodies (full-length human hnRNP-A1) with the Drosophila sequence. This allows us to assess the likelihood of cross-reaction of the antibody with the untested species. I did find an apparent analog, the product of the Dm gene Hrb87F (Synonym: hrp36).

Alignment of human hnRNP1 with this protein returns 57% identity. This suggests low potential for cross-reactivity (we look for 85% or better), but if your customer is still interested in trying one of these to IP Dm protein, I suggest ab5832, as there have been inconsistent results lately from ab10685. However, the antibody does not IP the hnRNP complex.

Customer reported receiving an empty vial of ab10685. Order# 98848. Lot# 10865.

ANSWER:

Thank you for your phone call and I apologize for the empty vial that you received. I'm sending you a free of charge replacement vial on order reference # 105847 to your attention. The item is currently out of stock with a delivery date estimated to be 18th October. You will receive email confirmations regarding shipping to keep you updated.

Please let me know if you have any additional questions or concerns.

The customer is using this antibody in Western blotting with squamous cell carcinoma samples and is seeing a band at 34 kDa. However, she has used this clone in the past (not from us, but from the original source) and consistently saw a band at 34 kDa but also a band above and below (a triplet). Is there any information regarding this?

ANSWER:

Thank you for your enquiry.

We have been informed by the source of ab10685 that the information supplied by the originator of this clone states a single band at approx 34 kDa. We have no report of other bands.

We hope this information helps, please do not hesitate to contact us again if you need further assistance,