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Anti-hnRNP A1 antibody [4B10] (ab10685)

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Overview

Product name

Anti-hnRNP A1 antibody [4B10]
See all hnRNP A1 products (5) ...

Description

Mouse monoclonal [4B10] to hnRNP A1

Tested applications

ICC/IF, IP, WB, ELISA, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Full length native protein partially purified.

Positive control

Hela cells in WB

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified antibody (from supernatant).

Clonality

Monoclonal

Clone number

4B10

Myeloma

Sp2/0

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other

  • Western blot - hnRNP A1 antibody [4B10] (ab10685)Western blot - hnRNP A1 antibody [4B10] (ab10685) image (enlarge)

  • Immunoprecipitation - hnRNP A1 antibody [4B10] (ab10685)Immunoprecipitation - hnRNP A1 antibody [4B10] (ab10685) image (enlarge)

  • Western blot - hnRNP A1 antibody [4B10] (ab10685)Western blot - hnRNP A1 antibody [4B10] (ab10685) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

Sequence similarities

Contains 2 RRM (RNA recognition motif) domains.

Post-translational
modifications

Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated.

Cellular localization

Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.

Target information above from: UniProt accessionP09651 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HNRNPA 1 antibody
  • Helix destabilizing protein antibody
  • Helix-destabilizing protein antibody
  • Heterogeneous nuclear ribonucleoprotein A1 antibody
  • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
  • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
  • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
  • hnRNP A1 antibody
  • hnRNP core protein A1 antibody
  • HNRNPA1 antibody
  • HNRPA1 antibody
  • MGC102835 antibody
  • Nuclear ribonucleoprotein particle A1 protein antibody
  • ROA1_HUMAN antibody
  • Single strand DNA binding protein UP1 antibody
  • Single strand RNA binding protein antibody
  • Single-strand RNA-binding protein antibody
  • syncrip antibody
see all

Database links

see all

Anti-hnRNP A1 antibody [4B10] images:

  Western blot - hnRNP A1 antibody [4B10] (ab10685)

Western blot - hnRNP A1 antibody [4B10] (ab10685)

All lanes : Anti-hnRNP A1 antibody [4B10] (ab10685)

Lane 1 : 20ug Jurkat cell lysate
Lane 2 : 20ug Jurkat cell lysate from cells transfected with hnRNP A1 siRNA


Predicted band size : 38.4 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)

  Immunoprecipitation - hnRNP A1 antibody [4B10] (ab10685)

Immunoprecipitation - hnRNP A1 antibody [4B10] (ab10685)

ab10685 used at a 1/100 dilution in Immunprecipitation. Whole cell lysate prepared from HeLaS3 cells used at 5 x 106 cells. Immunoprecipitation step performed using Dynabeads Protein G. Primary antibody incubated for 3 hours at 4°C. ab10685 used at a 1/2000 dilution in Western Blot.

This image is courtesy of an anonymous abreview.

See Abreview

  Western blot - hnRNP A1 antibody [4B10] (ab10685)

Western blot - hnRNP A1 antibody [4B10] (ab10685)

Anti-hnRNP A1 antibody [4B10] (ab10685) at 1/1000 dilution

Secondary
Anti-Mouse IgG AP Conjugate at 1/2000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 38.4 kDa

  Flow Cytometry-hnRNP A1 antibody [4B10](ab10685)

Flow Cytometry-hnRNP A1 antibody [4B10](ab10685)

Overlay histogram showing HeLa cells stained with ab10685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10685, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-hnRNP A1 antibody [4B10] (ab10685)

This product has been referenced in:

  • Van Summeren Aet al. Proteomics Investigations of Drug-Induced Hepatotoxicity in HepG2 Cells. Toxicol Sci 120:109-22 (2011). WB; Human.Read more (PubMed: 21163907) »
  • Clower CVet al. The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci U S A 107:1894-9 (2010). WB; Human.Read more (PubMed: 20133837) »

See all 9 publications for this product

Publishing research using ab10685? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"