Synthetic peptide derived from within residues 50 - 150 of Human hnRNP A2B1.
(Peptide available as ab31644.)
Our Abpromise guarantee covers the use of ab31645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35, 38 kDa (predicted molecular weight: 35 , 37 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
ab31645 staining hnRNP A2B1 in CHO cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour 30 minutes at 21°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Green - hnRNP, Red -alpha tubulin, Blue - nuclei.