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hnRNP C1 + C2 protein (Tagged-His Tag) (ab91927)

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Overview

  • Product namehnRNP C1 + C2 protein (Tagged-His Tag)See all hnRNP C1 + C2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 84-293 (210 amino acids) of Human hnRNP C1 + C2 with N terminal His tag; Predicted MWt 24 kDa (UniProt P07910-2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 117 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesLAAEPKVNRGKAGVKRSAAEMYGSSFDLDYDFQRDYYDRM YSYPARVPP PPPIARAVVPSKRQRVSGNTSRRGKSGFN SKSGQRGSSKSGKLKGDDLQ AIKKELTQIKQKVDSLLE NLEKIEKEQSKQAVEMKNDKSEEEQSSSSVK KDETNVK MESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEA EE GEDDRDSANGEDDS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91927 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C1C2Heterogeneous nuclear ribonucleoprotein C
        Heterogeneous nuclear ribonucleoprotein C (C1/C2)Heterogeneous nuclear ribonucleoproteins C1/C2HNRNPhnRNP C1 / hnRNP C2hnRNP C1/C2hnRNPCHNRPCHNRPC_HUMANMGC104306MGC105117MGC117353MGC131677Nuclear ribonucleoprotein particle C1 proteinNuclear ribonucleoprotein particle C2 proteinSNRPC
      see all
  • FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
  • Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localizationNucleus. Component of ribonucleosomes.

    • Target information above from: UniProt accession P07910 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    hnRNP C1 + C2 protein (Tagged-His Tag) images

    • SDS-PAGE - hnRNP C1 + C2 protein (Tagged-His Tag) (ab91927)
      SDS-PAGE - hnRNP C1 + C2 protein (Tagged-His Tag) (ab91927)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for hnRNP C1 + C2 protein (Tagged-His Tag) (ab91927)

    ab91927 has not yet been referenced specifically in any publications.

    Publishing research using ab91927 ? Please let us know so that we can cite the reference in this datasheet

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