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Overview

  • Product namehnRNP K protein (Tagged-His Tag)See all hnRNP K proteins and peptides ...
  • Protein descriptionSynthetic peptide, corresponding to amino acids 198-464 of hnRNP K (isoform 2) with N terminal His tag; 267 amino acids, 36kDa (29.4kDa predicted from sequence). UniProt ID = P61978.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 150 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYG GFTMMFDDRRGRPVGF PMRGRGGFDRMPPGRGGRPMPP SRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPL PPPP PPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWS PSEWQMAYEPQG GSGYDYSYAGGRGSYGDLGGPIITTQ VTIPKDLAGSIIGKGGQRIKQIRHESGASI KIDEPLEG SEDRIITITGTQDQIQNAQYLLQNSVKQYADVEGF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91867 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CSBPdC stretch binding proteinFLJ41122
        Heterogeneous nuclear ribonucleoprotein KhnRNP KHNRNPKHNRPKHNRPK_HUMANTransformation up regulated nuclear proteinTransformation up-regulated nuclear proteinTransformation upregulated nuclear proteinTUNP
      see all
  • FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    modifications    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.

    • Target information above from: UniProt accession P61978 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    hnRNP K protein (Tagged-His Tag) images

    • SDS-PAGE - hnRNP K protein (Tagged-His Tag) (ab91867)
      SDS-PAGE - hnRNP K protein (Tagged-His Tag) (ab91867)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for hnRNP K protein (Tagged-His Tag) (ab91867)

    ab91867 has not yet been referenced specifically in any publications.

    Publishing research using ab91867 ? Please let us know so that we can cite the reference in this datasheet

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