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Product Name
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hnRNP M1-M4 antibody [1D8]
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See all hnRNP M1-M4 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1D8] to hnRNP M1-M4
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Immunogen
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M19 fusion protein containing full-lengthhuman protein.
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Pig, Rb
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Specificity
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This antibody is specific for human RNP M1-M4.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: 1/75.
WB: 1/500. Detects a band of approximately 64-68 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
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Relevance
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Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript specific packaging and alternative splicing of pre mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA binding proteins with greatest similarity to a hypothetical RNA binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64 kDa subunit of cleavage stimulation factor, which is involved in 3’ end maturation of pre mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1D8
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Ascites
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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0.740 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this hnRNP M1-M4 antibody [1D8] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for hnRNP M1-M4 antibody [1D8] (ab9547)
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This product has been used in:
Datar KV et al. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins. Nucleic Acids Res 21:439-46 (1993). PubMed: 8441656
If you publish research using ab9547 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to hnRNP M1-M4
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hnRNP M1-M4 antibody [1D8] - more information
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